β-thalassemia major is expected to be fatal without early treatment with iron chelates and blood transfusions for the patient. Although lipid disorders and β-thalassemia are related, it is not clear how these problems developed. Although lipid abnormalities are linked to β-thalassemia, how these issues come about is unknown. Aim of the study: This is a cross-sectional study in which we will attempt to analyze and evaluate the lipid profile in β-Thalassemia patients' sera. Subjects and Methods: 175 participants were classified into two groups; the first involved 100 patients with β-thalassemia major (59 males and 41 females), aged 18 to 22 years. The second group involved 75 healthy individuals aged 18 to 32 (35 males and 40 females). Results: The S. cholesterol concentration was reduced, as were the low-density lipoprotein concentration and high-density lipoprotein, which were both significantly reduced, while the S. triglyceride concentration rose significantly in β-thalassemia major patients. Conclusion: The fluctuation of the level of blood lipids in patients with β-thalassemia major, although it has a downward trend from its typical concentrations, requires a pause because it may have health consequences on various levels, especially those directly related to lipids and their metabolic pathways.