Objective: Breast Cancer (BC) is the most common cancer worldwide and is the second leading cause of cancer deaths among women, its incidence continues to escalate. The development of BC is a multi-step process involving multiple cell types, early diagnosis of BC is one of the best approaches to prevent this disease. Methods: This case-control study included sixty women with breast cancer as well as 20 healthy women. The cases were selected using simple random sampling from Al-Amal National Hospital for Cancer Management. The present study performed to assess the levels of some parameters in BC women versus healthy women, which including CA15-3 marker, hematological parameters (including WBSs, RBCs, Hb, Platelets and ESR), liver function tests that included Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (ALP) kidney function tests (urea and creatinine) and some electrolytes (Cl-, K+, Na+ mmol/L). Results: The mean and Standard Deviation (SD) of the age of breast cancer patients are 51.81±11.323, while for healthy individuals, they are 30.25±4.756. The results of the statistical analysis showed significant differences (p = 0.004). The classification of patients by age into three groups showed that 31% were in the 25-45 age group, 35% in the 46-56 age group and 33.3% in the 57-77 age group. Regarding the assessment of studied parameters, the present study showed a significant (p = 0.032) increase in the levels of CA15-3 in women with breast cancer versus healthy women. As for hematological parameters, the statistical analysis showed significant differences only in RBCs, Hb and ESR: RBCs and Hb were significantly decreased (p = 0.038, p = 0.000, respectively) in breast cancer patients compared with controls, while ESR was significantly increased (p = 0.000). In terms of liver function tests, only ALP showed a significant (p = 0.000) increase in women with breast cancer patients as compared with control women; while AST and ALT showed non-significant differences. Regarding the assessment of kidney function tests and electrolytes, the statistical analysis showed that only urea increased significantly (p = 0.054) in patients, while creatinine and all studied electrolytes showed non-significant differences between patients and the control group. CA15-3 shows a significant inverse correlation with WBCs in breast cancer patients and with K+ only. No other significant correlations were found with the other studied parameters in either patients or healthy individuals. Conclusion: The current findings highlight the multifactorial nature of BC pathophysiology, where hematological, biochemical and metabolic shifts come together to shape cancer progression and prognosis.