In Africa and Madagascar's traditional medicine, Dombeya wallichii belonging to the family Malvaceae is claimed to possess powerful anti-hypertensive activity. However, as yet, the anti-hypertensive potential of Dombeya wallichii has not been investigated by scientifically controlled studies. This study was designed to investigate the anti-hypertensive potential of ethanolic extract of Dombeya wallichii. Phytochemical analysis of ethanolic extract of D. wallichii leaves and stem-root indicated that plant contain great amounts of phenolic and flavonoid compounds. Chemical constituents detected in Dombeya wallichii leaf extract (DWLE) were Chlorogenic acid, P-cumaric acid, Gallic acid, HB Acid, Sinapic acid, Salicylic acid and Benzoic acid. While Chlorogenic acid, P-cumaric acid, Sinapic acid, Vinallic acids were detected in Dombeya wallichii stem and root extract (DWSRE) through HPLC analysis. FTIR analysis of DWLE and DWSRE confirmed presence of different bioactive compounds containing a variety of functional groups, including alcohol and alkyl halide. As a result of DPPH, the ethanolic extract of plant displayed notable in vitro anti-oxidant activity. The plant extract was screened for its in vivo safety profile through acute oral toxicity study. Acute oral toxicity study was performed at the dose of 2000 mg/kg as a single oral dose. For the evaluation of diuretic study, 24 rats were divided into 4 groups each group contain 6 animals. First group served as a normal control, the second standard control was given furosemide (20 mg/kg), the third and fourth group served as a test groups. Diuretic activity was measured by giving DWLE and DWSRE ethanolic extract orally at a dose of 600 mg/kg to rats. All groups received 0.9 % N/S at the rate of 5 mL/100g of body weight. The cumulative urine output was measured at 6h, 12h and 24h time intervals. It was increased in DWLE group in contrast to DWSRE. Notably, Dombeya wallichii Leaf Extract (DWLE) and Dombeya wallichii Stem + Root Extract (DWSRE) demonstrated significant diuretic activity and had notable impacts on pH, Na+, K+ and Cl levels compared to the standard control (Furosemide) and normal saline. These findings suggest potential therapeutic applications for Dombeya wallichii extracts in modulating diuretic and electrolyte-related responses, but DWLE is more potent than DWSRE. When DWLE was compared DWSRE it showed statistically p < 0.05 (significant) results. These findings suggest potential therapeutic implications for Dombeya wallichii extracts in the context of renal function and electrolyte balance.