Objective: This study aimed to assess the level of knowledge regarding needle stick injuries among healthcare providers. Setting and Duration: The research was carried out in 25 primary healthcare centers located in Erbil city, Kurdistan Region, Iraq, over a period from March 2020 to August 2022. Methodology: A total of 687 healthcare providers, including nurses, physicians and paramedical staff, were enrolled in the study. Data were gathered through a structured questionnaire consisting of five items, specifically designed to evaluate knowledge related to needle stick injuries and their preventive measures. Results: Out of all participants, 402 (58.5%) were female and 285 (41.5%) were male. Nurses represented 41.5% of the total sample, while 39.9% of participants held diploma-level qualifications. Although more than half of the respondents had over ten years of professional experience, a significant majority 656 participants (96%) exhibited inadequate knowledge regarding needle stick injuries. Only 10.8% had previously received formal training on the subject. Additionally, 76.4% of participants showed limited awareness about the importance of hepatitis B vaccination as a preventive strategy. Conclusion: The study highlights a considerable deficiency in knowledge and preventive practices related to needle stick injuries among healthcare providers. There is a critical need for the implementation of standardized training programs and strengthening of occupational safety protocols to enhance awareness and minimize risks in healthcare environments.