Previous studies indicate that cardiac troponin I and cardiac troponin T exhibit diagnostic performance comparable to and potentially superior to, Creatine Kinase-MB (CK-MB) in identifying Acute Myocardial Infarction (AMI). These biomarkers may therefore serve as reliable alternatives to CK-MB in clinical detection. The accuracy of assay results can be ensured through the use of appropriate positive and negative controls included in diagnostic kits. The present study aims to assess myocardial cell and tissue injury by quantifying variations in cardiac enzyme levels and to examine the association between enzyme elevation and the extent of tissue damage across different age groups and between genders. Blood samples were obtained from 30 individuals at a Community Health Centre in Acharapakkam, comprising patients aged 30–50 years and 50–70 years. Each group included 15 participants, divided equally into experimental (myocardial infarction) and control (healthy) cohorts. Findings from this comparative analysis demonstrate a significant elevation in all measured cardiac enzymes among myocardial infarction patients, reflecting substantial myocardial cell damage. Enzyme levels were markedly higher in male patients compared to females. Additionally, individuals in the 50–70-year age group exhibited a greater degree of myocardial injury compared to the younger cohort.