Background: Infertility is a global health concern with multifaceted dimensions and has a significant impact on women’s reproductive health. This study examines infertility awareness, associated risk factors, and treatment options among women in District Mandi, Rusafa, while considering the unique sociocultural dynamics of the region. Materials and Methods: A cross-sectional survey was conducted from August to November 2023 among 400 women of reproductive age residing in District Mandi. Data were collected using a Google Form–based questionnaire that assessed demographic characteristics, knowledge of infertility, and attitudes toward infertility. The collected data were analyzed using Epi Info version 7 software, applying descriptive statistical methods. Results: Among the 400 respondents, 29.25% demonstrated very good knowledge, reflecting a strong understanding of infertility-related concepts. However, notable knowledge gaps were identified in specific areas, including preimplantation genetic testing (PGT) and fertility preservation, indicating the need for targeted educational interventions. Cultural influences were found to positively shape perceptions of infertility, while psychosocial factors highlighted the emotional burden associated with infertility. Conclusion: This study provides important insights into infertility awareness among women in District Mandi, Rusafa. Addressing identified knowledge gaps, understanding cultural influences, and recognizing psychosocial dimensions are essential components of comprehensive reproductive health interventions.