Background: Lung cancer represents a major global health challenge, highlighting the need for a comprehensive understanding of awareness and knowledge levels to support effective prevention and early detection strategies. This study explores lung cancer awareness within the specific context of District Johor Bahru, Malaysia, where regional and socio-cultural variations may influence awareness patterns. Materials and Methods: This cross-sectional survey study included 400 adult participants residing in District Johor Bahru. Data were collected using a Google Form–based questionnaire comprising socio-demographic variables and 20 knowledge-related questions on lung cancer. Based on predefined scoring criteria, participants were categorized into very good, good, fair, and poor knowledge levels. Data collection was conducted from August to October 2023 using online platforms. Results: The analysis demonstrated a diverse distribution of participants, with 33% from urban areas and 67% from rural areas. Overall, 25.5% of participants exhibited very good knowledge, 36% demonstrated good knowledge, while 38.25% showed fair to poor knowledge levels. Notable knowledge gaps were observed, particularly regarding lung cancer screening methods, types, and stages. These findings are consistent with global trends and emphasize the necessity for context-specific awareness interventions. Conclusion: This study provides valuable insights into lung cancer awareness in District Johor Bahru, identifying both existing strengths and areas requiring improvement. The findings support the implementation of targeted health communication strategies and community-based educational programs to address identified knowledge gaps. Strengthening awareness initiatives may contribute to earlier detection, improved clinical outcomes, and a reduced burden of lung cancer within this unique socio-cultural setting.